# US 10,440,907 B2 · Maize plants with improved disease resistance

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- Number: US 10,440,907 B2
- Document ID: US10440907B2
- Status: Granted
- Kind: B2
- Application number: 14/333,293
- Filed: 2014-07-16
- Published: 2015-01-22
- Granted: 2019-10-15
- Earliest claimed: 2013-07-17
- Assignees: Monsanto Technology LLC
- Applicants: Monsanto Technology LLC
- Inventors: George J. Baley; Juan Cruz; Hongwu Jia; Yule Pan; Hao Zhou
- Web page: https://patentgrep.com/patent/US10440907B2

## CPC

- A01H 1/1255: {for fungal resistance}
- A01H 1/045: {using molecular markers}
- A01H 5/10: Seeds
- A01H 6/4684: {Zea mays [maize]}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding corn plants containing one or more markers that are associated with resistance to fungi. The invention further includes germplasm and the use of germplasm containing at least one marker associated with resistance to Fusarium stalk rot (FSR) infection for introgression into elite germplasm in a breeding program, thus producing novel FSR resistant germplasm.

## Claims

### Claim 1

A method of obtaining a corn plant with enhanced Fusarium stalk rot resistance, said method comprising:
a) providing a population of corn plants;
b) obtaining a DNA sample from at least one corn plant within said population;
c) detecting in said DNA sample the presence of a Fusarium stalk rot resistance allele at a polymorphic locus within 5 cM of SEQ ID NO:93, wherein said locus comprises a nucleotide at position 151 of SEQ ID NO:93 that is associated with Fusarium stalk rot resistance, and wherein said nucleotide is an “A”;
d) selecting a corn plant from said population of corn plants based on the presence of the Fusarium stalk rot resistance allele;
e) crossing the plant comprising the Fusarium stalk rot resistance allele with a second, different corn plant to produce progeny plants wherein at least one progeny plant comprises the Fusarium stalk rot resistance allele and exhibits enhanced Fusarium stalk rot resistance when compared to a plant lacking said allele.

### Claim 2 (depends on 1)

The method of claim 1, wherein said segment is flanked by:
marker loci SEQ ID NO: 1 and SEQ ID NO: 100; or
marker loci SEQ ID NO: 90 and SEQ ID NO: 2.

### Claim 3 (depends on 1)

The method of claim 1, wherein said polymorphic locus comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, and SEQ ID NO: 100.

### Claim 4 (depends on 1)

The method of claim 1, further defined as comprising selecting from said population at least two plants, thereby forming a population of corn plants comprising said allele and enhanced Fusarium stalk rot resistance compared to a plant lacking said allele.

### Claim 5 (depends on 1)

The method of claim 1, wherein said Fusarium stalk rot resistance allele was introgressed into said population of corn plants from a starting plant or population of corn plants containing said allele.

### Claim 6 (depends on 1)

The method of claim 1, wherein producing the progeny plant comprises marker-assisted selection for Fusarium stalk rot resistance.

### Claim 7 (depends on 1)

The method of claim 1, wherein the progeny plant is an F2-F6 progeny plant.

### Claim 8 (depends on 1)

The method of claim 1, wherein producing the progeny plant comprises backcrossing.