US 10,435,755 B2 · Detecting esophageal disorders

Abstract

Provided herein is technology for esophageal disorder screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of esophageal disorders (e.g., Barrett's esophagus, Barrett's esophageal dysplasia, etc.). In addition, the technology provides methods, compositions and related uses for distinguishing between Barrett's esophagus and Barrett's esophageal dysplasia, and between Barrett's esophageal low-grade dysplasia, Barrett's esophageal high-grade dysplasia, and esophageal adenocarcinoma within samples obtained through endoscopic brushing or nonendoscopic whole esophageal brushing or swabbing using a tethered device (e.g. such as a capsule sponge, balloon, or other device).

Claims8

Dependent claims

A method for measuring the methylation level of no more than ten genetic markers at least including ELMO1 and CD1D comprising:

a) extracting genomic DNA from a biological sample of a human individual suspected of having or having Barrett's esophageal dysplasia and/or esophageal adenocarcinoma,
b) treating the extracted genomic DNA with bisulfite,
c) amplifying the bisulfite-treated genomic DNA with primers consisting of a pair of primers specific for ELMO1 and a pair of primers specific for CD1D, and
d) measuring the methylation level of no more than ten genetic markers at least including ELMO1 and CD1D by measuring the methylation of CpG sites in the genetic markers by methylation-specific PCR, quantitative methylation-specific PCR, methylation sensitive DNA restriction enzyme analysis or bisulfite genomic sequencing PCR.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/550,703Filed 23 Mar 2016 · us provisional application

Priority

27 Mar 2015

Published

US 2018/0037958 A18 Feb 2018

Granted

8 Oct 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12Q 1/6886
For diseases caused by alterations of genetic material: for cancer
C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
G01N 33/57557
For cancer: of other specific parts of the body, e.g. brain
C12Q 2600/112
Oligonucleotides characterized by their use: disease subtyping, staging or classification

Detecting esophageal disorders

Life of this patent

Detecting esophageal disorders

Life of this patent

Abstract

Claims8