Combination of biomarkers for detecting and evaluating a hepatic fibrosis

An in vitro method for determining the hepatic fibrosis score of a subject infected with one or more hepatitis viruses, characterized in that it comprises the following steps:

• i) in a sample which has been obtained from said subject, measuring the levels to which the selected genes are transcribed or translated, said selected genes comprising:
• SPP1, and
• at least one gene from among A2M and VIM, and
• at least one gene from among IL8, CXCL10 and ENG, and
• at least one gene selected from:
• IL6ST, p14ARF, MMP9, ANGPT2, CXCL11, MMP2, MMP7, S100A4, TIMP1, CHI3L1, COL1A1, CXCL1, CXCL6, IHH, IRF9 and MMP1, and
• ii) generating, by a computer comprising a processing unit, a hepatic fibrosis score, said hepatic fibrosis score being generated by comparing the measurement values of each of said selected genes obtained for said subject with their values, or with the distribution of their values, in reference cohorts which have been pre-established as a function of their hepatic fibrosis score, and classifying said subject as having a hepatic fibrosis score of those reference cohorts to which the subject has the highest probability of belonging.