Method for producing recombinant proteins using recombinant brevibacillus
AssigneeKaneka Corporation
InventorsHiroyuki Kanamaru, Teruaki Takesue, Osamu Odawara, Takeyuki Tsuchidate
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.
Claims12
A method for producing a recombinant protein, comprising:
- a high-temperature culture step of culturing in a liquid medium at 34° C. or higher and 40° C. or lower a Brevibacillus choshinensis bacterium that contains a gene encoding the recombinant protein;
- a temperature shifting step of reducing the temperature of the liquid medium prepared in the high-temperature culture step without replacing the liquid medium, and
- a low-temperature culture step of culturing the Brevibacillus choshinensis bacterium in the liquid medium at a temperature at 25° C. or higher and lower than 32° C., which is lower than the culturing temperature in the high-temperature culture step by at least 2° C.-8° C.
The method for producing a recombinant protein according to claim, 1 wherein the recombinant protein is a bioactive protein.
Dependencies are inferred from the claim text.