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US 10,435,693 B2Granted
Organic acid synthesis from C1 substrates
AssigneeAlliance for Sustainable Energy, LLC
InventorsCalvin A. Henard, Michael T. Guarnieri
Life of this patent
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Abstract
Presented herein are biocatalysts and methods for converting C1-containing materials to organic acids such as muconic acid or adipic acid.
Claims11
An engineered cell from the genus Methylomicrobium, comprising exogenously added genes encoding a 3-dehydroshikimate dehydratase (AroZ), a protocatechuic acid decarboxylase (AroY), and a catechol 1,2-dioxygenase (CatA);
- wherein the cell uses the AroZ, the AroY, and the CatA resulting from the expression of the exogenously added genes to convert methane to muconic acid.
Dependencies are inferred from the claim text.