Photothermal substrates for selective transfection of cells
AssigneeThe Regents of the University of California
InventorsPei-Yu E. Chiou, Ting-Hsiang S. Wu, Sheraz Kalim Butt, Michael A. Teitell
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Abstract
This invention provides novel tools for surgery on single cells and substrates/devices for delivery of reagents to selected cells. In certain embodiments the substrates comprise a surface comprising one or more orifices, where nanoparticles and/or a thin film is deposited on a surface of said orifice or near said orifice, where the nanoparticles and/or a thin film are formed of materials that heat up when contacted with electromagnetic radiation. In certain embodiments the pores are in fluid communication with microchannels containing one or more reagents to be delivered into the cells.
Claims25
A device for delivering an agent into a cell, said device comprising:
- a vessel comprising walls and a floor where the walls and/or floor comprise a plurality of orifices leading outside said vessel, wherein nanoparticles and/or a thin film of a material is disposed on said walls and/or floor at and/or near each of the orifices comprising said plurality of orifices, wherein said material is one that that heats up when illuminated by a laser or non-coherent light source;
- a laser or non-coherent light source directed to all or to a portion of the nanoparticles or thin film, wherein said laser or non-coherent light source is capable of heating said nanoparticles or thin film to a temperature sufficient to generate cavitation bubbles in a fluid in said vessel;
- and
- wherein said device is configured for delivering said agent into said vessel through one or more orifices comprising said plurality of orifices to selectively deliver said agent into cells disposed at or near said one or more orifices via the formation of cavitation bubbles when the nanoparticles or thin film at or near said one or more orifices is heated by said laser or non-coherent light source.
Dependencies are inferred from the claim text.