Antibodies to the surface of Toxoplasma gondii oocysts and methods of use thereof
AssigneeThe Regents of the University of California
InventorsHeather Fritz, Patricia Conrad
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Abstract
The present disclosure provides antibodies that bind the surface of Toxoplasma gondii oocysts, methods for using such antibodies and kits and devices for practicing such methods. Such antibodies, methods, kits and devices find use in detection of T. gondii oocysts and the isolation of such oocysts from samples including environmental samples, food-based samples, diagnostic samples, and the like.
Claims12
A method of specifically detecting an intact Toxoplasma gondii oocyst in a sample, the method comprising:
- a) contacting a sample suspected of containing a T. gondii oocyst with an IgG monoclonal antibody that specifically binds a protein on the outer wall of an intact T. gondii oocyst under conditions sufficient to form an immunocomplex of the antibody with the intact T. gondii oocyst;
- and
- b) specifically detecting the presence or absence of the immunocomplex comprising the antibody.
A method of specifically isolating an intact Toxoplasma gondii oocyst in a sample, the method comprising:
- a) contacting a sample suspected of containing a T. gondii oocyst with an IgG monoclonal antibody that specifically binds a protein on the outer wall of an intact T. gondii oocyst under conditions sufficient to form an immunocomplex of the antibody with the intact T. gondii oocyst;
- and
- b) specifically isolating the oocyst based on the binding of the antibody to the intact T. gondii oocyst.
Dependencies are inferred from the claim text.