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US 10,428,357 B2Granted
Preparation of (R)-3-hydroxybutyric acid or its salts by one-step fermentation
AssigneeNNB Nutrition USA, LLC
InventorsKylin Liao, Wenchao Fan
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Abstract
The subject invention relates to a process of preparing (R)-3-hydroxybutyric acid or a salt thereof by one-step fermentation with a nonpathogenic microorganism. The fermentation of (R)-3-hydroxybutyric acid was performed by supplying with certain carbon and nitrogen sources. These microorganisms include a Glutamic acid Bacterium HR057 strain or one type of genetically engineered Corynebacterium Glutamicum.
Claims1
A genetically engineered nonpathogenic microorganism,
- wherein the nonpathogenic microorganism is a Corynebacterium glutamicum strain deposited at the China General Microbiological Culture Collection Center under the accession number CGMCC No. 13111;
- wherein the nonpathogentica microorganism is capable of producing and accumulating (R)-3-hydroxybutyric acid in a fermentation broth during a fermentation process;
- and
- wherein the nonpathogenic microorganism has the following biotransformation capability:
- converting pyruvic acid and coenzyme A to acetyl-CoA, converting acetyl-CoA into acetoacetyl-CoA, converting acetoacetyl-CoA to acetoacetic acid, and then converting acetoacetic acid to (R)-3-hydroxybutyric acid.
2 more elements
Dependencies are inferred from the claim text.