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US 10,428,348 B2Granted
Reproduction of female sterility lines and its application in hybrid seed production
AssigneeExalt State Holdings Limited
InventorsNing Wei, Haodong Chen, Zhongcheng Liang
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Abstract
Provided in the present invention is a method for preparing hybrid rice, comprising the reproduction and maintenance of the female sterility line and the use thereof in preparing hybrid rice.
Claims15
A DNA construct, comprising:
- i) a first nucleotide sequence selected from the group consisting of OsFMS2 gene and OsFMS 1 gene;
- ii) a second nucleotide sequence selected from a group consisting of maize α-amylase gene, auxin gene, rot B gene, cytotoxin gene, diphtherin gene, DAM methylase gene, and PA gene;
- iii) a third nucleotide sequence selected from the group consisting of chloromycetin resistance gene, hygromycin resistance gene, streptomycin resistance gene, miramycin resistance gene, sulfonamide resistance gene, glyphosate resistance gene, glufosinate resistance gene, red fluorescence protein gene, cyan fluorescent protein gene, yellow fluorescent protein gene, luciferase gene, green fluorescent protein gene, an anthocyanin p1 gene, and blue fluorescent protein gene, and
- at least one regulatory nucleotide sequence that allows expression of the first, the second and the third nucleotide sequences in a plant,
- wherein the OsFMS2 gene has a nucleotide sequence selected from the group consisting of SEQ ID NOS:1 and 2, and
- wherein the OsFMS1 gene has a nucleotide sequence selected from the group consisting of SEQ ID NOS:17 and 18.
3 more elements
A DNA construct, comprising:
- i) a first nucleotide sequence selected from the group consisting of OsFMS2 gene and OsFMS 1 gene;
- ii) a second nucleotide sequence selected from a group consisting of maize α-amylase gene, auxin gene, rot B gene, cytotoxin gene, diphtherin gene, DAM methylase gene, and PA gene;
- iii) a third nucleotide sequence selected from the group consisting of chloromycetin resistance gene, hygromycin resistance gene, streptomycin resistance gene, miramycin resistance gene, sulfonamide resistance gene, glyphosate resistance gene, glufosinate resistance gene, red fluorescence protein gene, cyan fluorescent protein gene, yellow fluorescent protein gene, luciferase gene, green fluorescent protein gene, an anthocyanin p1 gene, and blue fluorescent protein gene;
- a fourth nucleotide sequence, wherein the fourth nucleotide sequence is operably linked to the first nucleotide sequence and drives the first nucleotide sequence to express in a female organ of the plant;
- a fifth nucleotide sequence, wherein the fifth nucleotide sequence is operably linked to the second nucleotide sequence and drives the second nucleotide sequence to express in male gametes of the plant;
- and
- a sixth nucleotide sequence, wherein the sixth nucleotide sequence is a promoter that drives the third nucleotide sequence to express specifically in seeds or endosperm,
- wherein the OsFMS2 gene has a nucleotide sequence selected from the group consisting of SEQ ID NOS:1 and 2, and
- wherein the OsFMS1 gene has a nucleotide sequence selected from the group consisting of SEQ ID NOS:17 and 18.
6 more elements
Dependencies are inferred from the claim text.