Nucleotide sequences and corresponding polypeptides conferring modulated growth rate and biomass in plants grown in saline conditions
AssigneeCeres, Inc.
InventorsFasong Zhou, Kenneth A. Feldmann, Julissa Sosa
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Abstract
The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.
Claims8
A food or feed product comprising a tissue from a transgenic plant, wherein the transgenic plant and said tissue comprise a recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule comprises:
- (a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 154, and wherein expression of the nucleotide sequence in a plant increases salt stress tolerance as compared to a control plant of the same species lacking said nucleotide sequence;
- (b) a nucleotide sequence comprising the polynucleotide sequence of SEQ ID NO:
- 153, wherein the polynucleotide sequence encodes the protein of SEQ ID NO:154;
- or
- (c) a nucleotide sequence hybridizing to the full length complementary sequence of the nucleotide sequence as set forth in SEQ ID NO:
- 153 under high stringency conditions which comprise (i) hybridization at 42° C. in 50% formamide, 6×SSC or 6×SSPE, 0.05% Blotto or 5×Denhardt's Reagent, 100 g/ml denatured salmon sperm DNA, 0.05% SDS and washing at 65° C. first in 2×SSC, 0.1% SDS for at least 30 min to one hour and subsequently in 0.1×SSC, 0.5% SDS for at least 30 min to one hour;
- or (ii) hybridization at 65° C. in 6×SSC or 6×SSPE, 0.05% Blotto or 5×Denhardt's Reagent, 100 μg/ml denatured salmon sperm DNA, 0.05% SDS and washing at 65° C. first in 2×SSC, 0.1% SDS for at least 30 min to one hour, and subsequently in 0.1×SSC, 0.5% SDS for at least 30 min to one hour and wherein said hybridizing nucleotide sequence encodes a protein which has the functional activity of SEQ ID NO:
- 154;
- wherein said nucleotide sequence is operably linked to a heterologous promoter;
- wherein the protein encoded by the nucleotide sequence is expressed in the transgenic plant;
- and
- wherein expression of the protein encoded by said nucleotide sequence in the transgenic plant increases salt stress tolerance as compared to a control plant of the same species that does not comprise the recombinant nucleic acid molecule.
Dependencies are inferred from the claim text.