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US 10,428,327 B2Granted
Compositions and methods for enhancing homologous recombination
AssigneeLife Technologies Corporation
InventorsXiquan Liang, Robert Potter, Namritha Ravinder
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Abstract
The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.
Claims12
A method for performing homologous recombination, the method comprising:
- (a) generating a double-stranded break in a nucleic acid molecule present inside a cell to produce a cleaved nucleic acid molecule, and
- (b) contacting the cleaved nucleic acid molecule generated in (a) with a partially double-stranded donor nucleic acid molecule,
- wherein the cleaved nucleic acid molecule and the donor nucleic acid molecule each contain matched termini on at least one end,
- wherein the matched termini on at least one end of the cleaved nucleic acid molecule and on at least one end the donor nucleic acid molecule is at least ten nucleotides in length,
- wherein the matched terminus on at least one end of the cleaved nucleic acid molecule is double-stranded and the matched terminus on at least one end of the donor nucleic acid molecule comprises a 3′ single-stranded overhang.
2 more elements
Dependencies are inferred from the claim text.