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US 10,426,823 B2Granted
Methods for constructing antibiotic resistance free vaccines
AssigneeThe Trustees of the University of Pennsylvania
InventorsYvonne Paterson, Thorsten Verch
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Abstract
The present invention provides Listeria vaccine strains that express a heterologous antigen and a metabolic enzyme, and methods of generating same.
Claims18
A composition comprising an antibiotic resistance gene-free plasmid, said plasmid comprising:
- i) a first nucleic acid sequence encoding a polypeptide, wherein said polypeptide is a fusion protein comprising a heterologous antigen and a non-hemolytic Listeriolysin (LLO) protein or N-terminal fragment thereof, and ii) a second nucleic acid sequence encoding a metabolic enzyme.
Dependencies are inferred from the claim text.