Intergenic sites between conserved genes in the genome of modified vaccinia ankara (MVA) vaccinia virus
AssigneeThe U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorsBernard Moss, Linda S. Wyatt, Patricia L. Earl
Life of this patent
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Abstract
The present invention relates to new insertion sites useful for the integration of exogenous sequences into an intergenic region (IGR) of a vaccinia virus genome, where the IGR is located between or is flanked by two adjacent open reading frames (ORFs) of the vaccinia virus genome, and where the ORFs correspond to conserved genes, and to related plasmid vectors useful to insert exogenous DNA into the genome of a vaccinia virus, and further to recombinant vaccinia viruses comprising an exogenous sequence inserted into said new insertion site as a medicine or vaccine.
Claims16
A plasmid vector comprising a cloning site flanked by:
- a) a first nucleic acid sequence derived from at least a portion of the 3′ terminus of a first essential ORF from vaccinia virus genome;
- and
- b) a second nucleic acid sequence derived from at least a portion of the 3′ terminus of a second essential ORF from vaccinia virus genome;
- wherein the first and second essential ORFs are adjacent to one another in the vaccinia virus genome;
- wherein the first and second essential ORFs are oriented 3′ end-to-3′ end in the vaccinia virus genome;
- and,
- wherein the first and second nucleic acid sequences are capable of effecting homologous recombination with the genome of an MVA virus.
A plasmid vector comprising an intergenic region (IGR) flanked by:
- a) a first nucleic acid sequence derived from at least a portion of one end of a first essential ORF from a vaccinia virus;
- and,
- b) a second nucleic acid sequence derived from at least a portion of one end of a second essential ORF from a vaccinia virus;
- wherein the first and second essential ORFs are adjacent to one another in the vaccinia virus genome;
- wherein the end of the first essential ORF and the end of second essential ORF are proximal to one another in the vaccinia virus genome;
- wherein the first and second nucleic acid sequences are capable of homologous recombination with an MVA virus genome;
- and,
- wherein the IGR comprises the IGR of the first and second essential ORFs in the vaccinia virus genome.
Dependencies are inferred from the claim text.