Apparatus and method for culturing cells and tissue
AssigneeIonoptix LLC
InventorsPeter G. W. Lee, Douglas L. Tillotson, Richard T. Udale, Katherine L. Barber
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Abstract
A method of culturing cells or tissue comprises: (a) disposing cells or tissue in the cavity of a deformable cell culture container; and (b) applying synchronized chronic electrical stimulation and stretch to the cells or tissue in the deformable culture cell container, wherein the synchronized chronic electrical stimulation is applied in the form of pulses at a frequency of from 0.010-99 Hz and a duration of from 0.4 to 24 ms; and wherein stretch cycles are applied to the cells or tissue at a frequency of from 0.010 to 15 Hz.
Claims13
A method of culturing cells or tissue comprising:
- (a) disposing cells or tissue in a cavity of a deformable cell culture container;
- and (b) applying synchronized chronic electrical stimulation and stretch to the cells or tissue in the deformable culture cell container, wherein the synchronized chronic electrical stimulation is applied in the form of pulses at a frequency of from 0.010-99 Hz and a duration of from 0.4 to 24 ms;
- and wherein stretch cycles are applied to the cells or tissue at a frequency of from 0.010 to 15 Hz, and wherein a controller is provided which internally generates a reference signal and wherein the chronic electrical stimulation is synchronized to the reference signal and chronic stretch cycles are synchronized to the reference signal, thereby synchronizing the chronic electrical stimulation to chronic stretch applied to the cells or issue, and wherein the controller is programmed to provide both a reference signal frequency and a predetermined temporal relationship between recurring electrical stimulation and recurring stretch.
A method of culturing cells or tissue comprising:
- (a) disposing cells or tissue in a cavity of a deformable cell culture container;
- and
- (b) applying synchronized chronic electrical stimulation and stretch to the cells or tissue in the deformable culture cell container, wherein the synchronized chronic electrical stimulation is applied in the form of pulses at a frequency of from 0.010-99 Hz and a duration of from 0.4 to 24 ms;
- and wherein stretch cycles are applied to the cells or tissue at a frequency of from 0.010 to 15 Hz, and wherein a controller is provided which internally generated a reference signal and wherein the chronic electrical stimulation is synchronized to the reference signal and chronic stretch cycles are synchronized to the reference signal, thereby synchronizing the chronic electrical stimulation to chronic stretch applied to the cells or tissue, and wherein the controller is programmed to provide both a reference signal frequency and a predetermined temporal relationship between recurring electrical stimulation and recurring stretch;
- and wherein stretch is applied to the cell or issue in stretch cycles comprising a plurality of static phases where a stretch level is maintained and a plurality of dynamic phases where the deformable cell culture container is contracted or lengthened.
Dependencies are inferred from the claim text.