Engineered proteins with a protease cleavage site
AssigneeGreenLight Biosciences, Inc.
InventorsWilliam Jeremy Blake, Drew S. Cunningham
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Abstract
Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence, wherein the recombinant proteins can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
Claims8
A recombinant protein comprising a transketolase A polypeptide, wherein the transketolase A polypeptide has at least 95% identity to SEQ ID NO:
- 63 and has a human rhinovirus 3C protease recognition sequence located between amino acid positions corresponding to 637 and 638 of SEQ ID NO:
- 63.
A nucleic acid comprising the sequence of SEQ ID NO:
- 60, said sequence encoding the protein of SEQ ID NO:
- 66.
Dependencies are inferred from the claim text.