Interleukin-31 monoclonal antibody
AssigneeZoetis Services LLC
InventorsAndrea Joy Gonzales, Gregory Fici, Gary Francis Bammert, Steven Alan Dunham
Life of this patent
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Abstract
A cell-based assay for assessing the functional activity of a canine or feline IL-31 inhibitor candidate is provided. Such a canine or feline IL-31 inhibitor candidate can be an isolated antibody that specifically binds to at least one of canine Interleukin-31 (IL-31) or feline IL-31. Such antibodies can be in the form of diagnostic and/or veterinary compositions useful for treating a pruritic and/or allergic condition in dogs or cats.
Claims29
A cell-based assay for assessing the functional activity of a canine or feline IL-31 inhibitor candidate, comprising:
- providing cells that endogenously express receptors for and are responsive to canine or feline IL-31;
- incubating canine or feline IL-31 in the presence of a composition comprising an IL-31 inhibitor candidate or a vehicle control;
- treating the cells with the incubated canine or feline IL-31;
- measuring the direct phosphorylation of a protein induced by the canine or feline IL-31 treatment;
- and
- determining whether the composition comprising the canine or feline IL-31 inhibitor candidate inhibited the canine or feline IL-31 induced direct protein phosphorylation relative to the vehicle control.
A cell-based assay for assessing the functional activity of a canine or feline IL-31 inhibitor candidate,
- comprising:
- providing canine DH82 monocyte cells that endogenously express receptors for and are responsive to canine or feline IL-31;
- incubating canine or feline IL-31 in the presence of a composition comprising an IL-31 inhibitor candidate or a vehicle control;
- treating the DH82 monocyte cells with the incubated canine or feline IL-31;
- measuring a biological activity in the DH82 monocyte cells induced by the canine or feline IL-31 treatment;
- and
- determining whether the composition comprising the canine or feline IL-31 inhibitor candidate inhibited the canine or feline IL-31 induced biological activity relative to the vehicle control.
Dependencies are inferred from the claim text.