Scaffold for tissue growth and repair
AssigneeNew Jersey Institute of Technology
InventorsTreena Arinzeh, George Collins, Yee-Shuan Lee
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Abstract
Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.
Claims14
An electroactive structure for growing and differentiating a differentiable cell comprising a three dimensional matrix of electro spun fibers;
- wherein
- the electro spun fibers are formed by electrospinning a biocompatible synthetic piezoelectric polymer at an electric potential of between about 15 kV and about 30 kV;
- wherein the electro spun fibers comprise a higher β phase content than electro spun fibers formed by electrospinning the biocompatible synthetic piezoelectric polymer at an electric potential of less than about 15 kV;
- wherein the electro spun fibers are annealed, wherein annealing of the electro spun fibers further increases the β phase content and enhances piezoelectric characteristics;
- wherein the matrix of electro spun fibers forms a scaffold for supporting cell growth and differentiation;
- and
- wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into a cell with either an osteogenic or chondrogenic phenotype.
An electro active structure for growing and differentiating a differentiable cell comprising a three dimensional matrix of electro spun fibers;
- wherein
- the electro spun fibers are formed by electrospinning a biocompatible synthetic piezoelectric polymer selected from the group consisting of a polyvinylidene fluoride (PVDF) homopolymer, a trifluoroethylene homopolymer (TrFE) and a poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer at an electric potential of between about 15 kV and about 30 kV;
- wherein the electro spun fibers comprise a higher β phase content than electro spun fibers formed by electrospinning the biocompatible synthetic piezoelectric polymer at an electric potential of less than about 15 kV;
- wherein the electro spun fibers are annealed, wherein annealing of the electro spun fibers further increases the β phase content and enhances piezoelectric characteristics;
- wherein the matrix of electro spun fibers forms a scaffold for supporting cell growth and differentiation;
- and
- wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype.
Dependencies are inferred from the claim text.