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US 10,420,833 B2Granted
Combinatorial platform for the display of surface adjuvants and antigens
AssigneeBoard of Regents, The University of Texas System
InventorsM. Stephen Trent, Joseph M. Boll
Life of this patent
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Abstract
Engineered bacteria are provided that produce modified lipid A and a polypeptide or polysaccharide antigens. In some aspects, immunogenic compositions are provided comprising a modified a lipid A and a polypeptide or polysaccharide antigen.
Claims12
An isolated engineered bacteria strain comprising
- (a) at least one expression vector comprising at least one polynucleotide encoding a lipid A modification enzyme, wherein the gene encoding a lipid A modification enzyme is selected from the group consisting of lpxE, lpxF, lpxO, lpxR, pagL, and pagP;
- and
- (b) at least one expression vector comprising at least one polynucleotide encoding a polysaccharide antigen biosynthesis protein,
- wherein the engineered bacteria strain produces the polysaccharide antigen covalently conjugated to a modified lipid A;
- and
- wherein the engineered bacteria strain:
- (i) comprises a deletion of the Eck1673 gene;
- (ii) comprises at least lpxE, lpxO, pagL and pagP;
- (iii) produces the Vibrio cholerae O antigen, Salmonella typhimurium O antigen, or Shigella spcs O antigen antigen covalently conjugated to a modified lipid A;
- or
- (iv) produces the Streptococcus pneumonia capsule, Staphylococcus aureus capsule, or Neisseria meningitidis capsule antigen covalently conjugated to a modified lipid A.
8 more elements
Dependencies are inferred from the claim text.