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US 10,420,803 B2Granted
Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
AssigneeJanssen Biotech, Inc.
InventorsSebastian Rieck, Alireza Rezania
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Abstract
Cell populations of intestinal midgut endoderm cells and methods of generating the cells expressing markers characteristic of intestinal endoderm lineage are disclosed. Methods of treating conditions such as diabetes are also disclosed.
Claims9
A method of producing a population of intestinal midgut endoderm cells, comprising:
- culturing human pluripotent stem cells in a first culture media containing GDF-8 and a GSK3β inhibitor compound to definitive endoderm cells;
- culturing the definitive endoderm cells in a second culture media containing ascorbic acid and FGF7 to primitive gut tube cells;
- and
- culturing the primitive gut tube cells in a third culture media containing retinoic acid and BMP2 or BMP4 to intestinal midgut endoderm cells,
- wherein a population of substantially intestinal midgut endoderm cells is produced.
2 more elements
A method of producing a population of intestinal midgut endoderm cells, comprising:
- culturing human pluripotent stem cells in a first culture media containing GDF-8 and a GSK3β inhibitor compound to definitive endoderm cells;
- culturing the definitive endoderm cells in a second culture media containing ascorbic acid and FGF7 to primitive gut tube cells;
- and
- culturing the primitive gut tube cells in a third culture media containing retinoic acid and BMP2 or BMP4 to intestinal midgut endoderm cells,
- wherein the intestinal midgut endoderm cells form and remain stable as a monolayer.
2 more elements
Dependencies are inferred from the claim text.