Device and method for generating calibration factors for use in determining biological indicator levels in tissue

A system including an electronic device and a hardware server, the system configured to generate at least one calibration factor for use in determining optical density and comprising:

• a plurality of emitters, each having at least one light source, each of the emitters configured to emit light;
• a detector configured to receive light and transmit data representative of the received light;
• at least two of the plurality of emitters are spaced at different distances from the detector;
• a processor coupled to the at least one emitter and the detector;
• a non-transitory storage medium coupled to the processor and configured to store instructions to cause the device to repeat the following for each of the light sources:
• emit light from one of the light sources of one of the emitters at a predetermined current towards an object that has a known optical density;
• detect, at the detector, a portion of the emitted light corresponding to the predetermined current;
• calculate, at the processor, a corresponding calibration factor based on the detected portion of emitted light through the object;
• store the corresponding calibration factor in the non-transitory storage medium.