Devices for the detection of multiple analytes in a sample

A method for the detection of multiple nucleic acid analytes in a fluid sample, the method comprising:

• contacting a device with a fluid sample suspected of comprising one or more nucleic acid analytes of interest, each nucleic acid analyte of interest bound to a haptenic complementary nucleotide sequence, the fluid sample comprising a labeled control reagent, the device comprising a matrix defining an axial flow path, the matrix comprising:
• i) a sample receiving zone at an upstream end of the axial flow path that receives the fluid sample,
• ii) a label zone positioned within the axial flow path and downstream from the sample receiving zone, said label zone comprising a mobilizable signal producing component that binds each nucleic acid analyte of interest bound to its haptenic complementary nucleotide sequence,
• iii) at least two test zones positioned within the axial flow path and downstream from the label zone, wherein each test zone comprises a restraining component that restrains each nucleic acid analyte of interest bound to its haptenic complementary nucleotide sequence, and
• iv) a control zone positioned within the axial flow path and downstream from the label zone, the control zone comprising an immobilized member of a specific binding pair that binds with the labeled control reagent;
• and
• inspecting the at least two test zones for signal producing component captured by the restraining component at each of the at least two test zones, where presence of the signal producing component at a test zone indicates presence of the nucleic acid analyte of interest bound to its haptenic complementary nucleotide sequence.