Method for producing steviol and steviol glycoside using AOBGL3 homolog
AssigneeSuntory Holdings Limited
InventorsMisa Ochiai, Eiichiro Ono
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Abstract
The present invention provides a method for preparing a steviol glycoside and/or steviol comprising reacting glycosidase AOBGL1 and/or AOBGL3 or a variant thereof with a steviol glycoside having at least one monoglucoside bond and/or monoglucosyl ester bond, thereby cleaving said monoglucoside bond and/or monoglucosyl ester bond.
Claims13
A method for preparing a steviol glycoside and/or steviol comprising reacting a protein selected from the group consisting of proteins (a) to (c) shown below with a steviol glycoside having at least one monoglucoside bond and/or monoglucosyl ester bond, thereby cleaving said monoglucoside bond and/or monoglucosyl ester bond:
- (a) a protein consisting of the amino acid sequence of SEQ ID NO:
- 3 or 4;
- (b) a protein having an amino acid sequence having 90% or more sequence identity to the amino acid sequence of SEQ ID NO:
- 3 or 4, and having an activity to cleave a monoglucoside bond and/or monoglucosyl ester bond of a steviol glycoside;
- and
- (c) a protein comprising the amino acid sequence of SEQ ID NO:
- 3 or 4.
Dependencies are inferred from the claim text.