Modified microorganism for the optimized production of 2,4-dihydroxybutyrate
AssigneeMetabolic Explorer
InventorsPhilippe Soucaille, Gwénaëlle Bestel-Corre, Laurence Dumon-Seignovert
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Abstract
The present invention relates to a genetically modified microorganism for the production of 2,4-dihydroxybutyrate, by metabolic transformation of xylose via the 1,2,4-butanetriol intermediate. The invention also relates to a method for the production of 2,4-dihydroxybutyrate by culturing said genetically modified microorganism in a fermentation medium and recovering 2,4-DHB from said medium.
Claims10
A genetically modified Escherichia for the production of 2,4-dihydroxybutyrate (2,4-DHB) by converting xylose into 1,2,4-butanetriol, wherein said Escherichia is further genetically modified for:
- i) an overexpression of at least one gene encoding EC 1.1.1 enzyme which is NAD(P)+dependent, thereby oxidizing 1,2,4-butanetriol into 2,4-dihydroxybutanal;
- ii) an overexpression of at least one gene encoding EC 1.2.1 enzyme which is NAD(P)+dependent, thereby oxidizing 2,4-dihydroxybutanal into 2,4-dihydroxybutyrate;
- and
- iii) a deletion and/or attenuation of at least one of the following genes:
- a gene encoding a xylose isomerase,
- a gene encoding a xylulose kinase,
- a gene encoding a 3-deoxy-D-glycero-pentulosonate aldolase,
- a gene encoding a keto-acid dehydrogenase, and
- any combination thereof, and
- wherein said E. coli produces at least 5 mg/L of 2,4-DHB.
Dependencies are inferred from the claim text.