Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high throughput screening

A pseudotyped lentiviral vector particle comprising a fusion (F) protein and a hemagglutinin (H) protein of a morbillivirus,

• wherein the cytoplasmic portions of said F and H proteins are truncated by deletion of amino acid residues from said cytoplasmic portions, and
• wherein the truncated cytoplasmic portion of the F protein comprises at least 1 positively charged amino acid residue and no more than 9 consecutive amino acid residues as counted from the N-terminal end of the cytoplasmic portion of the F protein, the truncated cytoplasmic portion of the H protein is truncated to allow efficient pseudotyping and has fusion support function,
• wherein the truncated cytoplasmic portion of the H protein comprises at least 13 and no more than 19 consecutive amino acid residues as counted from the C-terminal end of the cytoplasmic portion of the H protein plus an additional methionine at the N-terminus,
• wherein the amount of truncated F protein is 250-750% higher than the amount of truncated H protein,
• wherein the morbillivirus is the Edmonston strain of measles virus,
• wherein said vector particle is capable to transduce cells expressing at least one of CD46 and SLAM or is capable to selectively transduce cells expressing SLAM,
• wherein said pseudotyped lentiviral vector particle selectively transfers a gene coding for a cytotoxic protein or an immune-stimulatory factor or an immune stimulatory protein or a functional copy of a defective or mutated gene in a patient suffering from an inherited disease into a target cell for the preparation of a medicament.