Tissue specific reduction of lignin
AssigneeThe Regents of the University of California
InventorsDominique Loque, Aymerick Eudes
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Abstract
The present invention provides an expression cassette comprising a polynucleotide that encodes a protein that diverts a monolignol precursor from a lignin biosynthesis pathway in the plant, which is operably linked to a heterologous promoter. Also provided are methods of engineering a plant having reduced lignin content, as well as plant cells, plant parts, and plant tissues from such engineered plants.
Claims15
A method of engineering a plant having reduced lignin content, the method comprising:
- introducing into the plant an expression cassette comprising a polynucleotide that encodes a bacterial dehydroshikimate dehydratase or a Podospora anserina dehydroshikimate dehydratase (DsDH), wherein the polynucleotide is operably linked to a heterologous promoter, wherein the promoter is a secondary cell wall-specific promoter, a fiber cell-specific promoter, or a promoter from a gene in the lignin biosynthesis pathway;
- and
- culturing the plant under conditions in which the bacterial dehydroshikimate dehydratase is expressed.
A plant cell comprising a polynucleotide that encodes a bacterial dehydroshikimate dehydratase or a Podospora anserina dehydroshikimate dehydratase (DsDH), wherein the polynucleotide is operably linked to a heterologous promoter, wherein the promoter is a secondary cell wall-specific promoter, a fiber cell-specific promoter, or a promoter from a gene in the lignin biosynthesis pathway.
Dependencies are inferred from the claim text.