US 10,415,046 B2Granted

Precision gene targeting to a particular locus in maize

AssigneeDow AgroSciences LLC

InventorsW. Michael Ainley, James W. Bing, David R. Corbin, Steven L. Evans, Joseph F. Petolino, Lakshmi Sastry-Dent, Steven A. Thompson, Steven R. Webb, Mary E. Welter, Ning Zhou

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Abstract

The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Claims13

Dependent claims

A method for integrating one or more exogenous nucleic acid sequences into the genome of a maize cell having an E32 locus, said method comprising the steps of:

making a double-stranded cleavage in the E32 locus using a site specific zinc finger nuclease, wherein the site specific zinc finger nuclease comprises three or more zinc finger motifs selected from the group consisting of SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, and SEQ ID NO:52;
and
ligating a polynucleotide comprising one or more exogenous nucleic acid sequences into the cleavage using a ligase or a recombinase, wherein the polynucleotide is integrated into the cleavage.

Dependencies are inferred from the claim text.

Facts

Application: 15/338,888Filed 31 Oct 2016 · continuation
Priority: 13 Dec 2012
Published: US 2017/0044558 A116 Feb 2017
Granted: 17 Sep 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8213
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…
C12N 9/1241
Transferring phosphorus containing groups, e.g. kinases: nucleotidyltransferases
C12Y 207/07047
Nucleotidyltransferases: streptomycin 3''-adenylyltransferase
C12N
Microorganisms or enzymes

Family

Application61/736,856Us provisional application · filed 13 Dec 2012
Application61/820,231Us provisional application · filed 7 May 2013
Application14/105,563Parent of this continuation · filed 13 Dec 2013
US 2017/0044558 A1Pre-grant publication · published 16 Feb 2017
US 2014/0173783 A1Published application
US 10,415,046 B2This patent

From the US filing history. Foreign family members aren’t listed.