Light-emitting versions of the monoclonal antibody to C3D (MAB 3D29) for imaging
AssigneesThe Regents of the University of Colorado, a body corporate, The Johns Hopkins University
InventorsMartin G. Pomper, Catherine A. Foss, Joshua M. Thurman, V. Michael Holers
Life of this patent
Estimating the term…
Abstract
The presently disclosed subject matter provides compositions and kits comprising light-emitting versions of the monoclonal antibody to C3d (mAB 3d29) for imaging and methods of use thereof for detecting infectious and inflammatory cells in vivo. The presently disclosed subject matter also provides methods for detecting and/or monitoring a Mycobacterium tuberculosis (M. tuberculosis) infection in a subject, as well as methods of treating a M. tuberculosis infection in a subject.
Claims21
A method for detecting a Mycobacterium tuberculosis (M. tuberculosis) infection in a subject, the method comprising:
- (a) administering to a subject an effective amount of a monoclonal antibody which binds to C3d in the subject, wherein the monoclonal antibody is conjugated to an imaging tag;
- and
- (b) detecting a signal generated by the imaging tag upon binding to C3d in the subject to detect the location of the M. tuberculosis infection in the subject.
A method of treating a M. tuberculosis infection in a subject in need thereof, the method comprising:
- (a) administering to a subject an effective amount of a monoclonal antibody which binds to C3d, wherein the monoclonal antibody is conjugated to an imaging tag, and wherein the monoclonal antibody binds to infected tissue in the subject;
- and
- (b) detecting a signal generated by the imaging tag to detect the location of the M. tuberculosis infection in the subject;
- and
- (c) administering to the subject an effective amount of an antibiotic an anti-inflammatory agent, or a combination thereof.
A method for detecting a Mycobacterium tuberculosis (M. tuberculosis) infection in a subject, the method comprising:
- (a) administering to a subject an effective amount of a monoclonal antibody or antibody-binding fragment thereof which binds to C3d in the subject, wherein the monoclonal antibody or antibody-binding fragment thereof is conjugated to an imaging tag;
- and
- (b) detecting a signal generated by the imaging tag upon binding to C3d in the subject to detect the location of the M. tuberculosis infection in the subject wherein the monoclonal antibody or antibody-binding fragment thereof which binds to C3d in the subject is a monoclonal antibody selected from the group consisting of the following:
- (i) a monoclonal antibody produced by the hybridoma cell line 3d-9a/25 deposited with the American Type Culture Collection (ATCC) as deposit number PTA-10998;
- (ii) a monoclonal antibody produced by the hybridoma cell line 3d-8b/2 deposited with the ATCC as deposit number PTA-10999;
- (iii) a monoclonal antibody produced by the hybridoma cell line 3d-29/5/2 deposited with the ATCC as deposit number PTA-11000;
- (iv) a monoclonal antibody produced by the hybridoma cell line 3d-10/14/1 deposited with the ATCC as deposit number PTA-11010;
- (v) a monoclonal antibody produced by the hybridoma cell line 3d-11/14 deposited with the ATCC as deposit number PTA-11011;
- (vi) a monoclonal antibody produced by the hybridoma cell line 3d-15A9 deposited with the ATCC as deposit number PTA-11012;
- (vii) a monoclonal antibody produced by the hybridoma cell line 3d-3/28/4 deposited with the ATCC as deposit number PTA-11025;
- (viii) a monoclonal antibody produced by the hybridoma cell line 3d-16/3/3 deposited with the ATCC as deposit number PTA-11026;
- and
- (ix) a monoclonal antibody produced by the hybridoma cell line 3d-31/A6/9 deposited with the ATCC as deposit number PTA-11027.
Dependencies are inferred from the claim text.