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US 10,408,843 B2Granted
Compositions and methods for detecting S-nitrosylation and S-sulfinylation
AssigneeThe Regents of the University of Michigan
InventorsBrent R. Martin, Jaimeen D. Majmudar
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Abstract
The present invention relates to methods for detecting protein S-sulfinylation and S-sulfinylation within thiol groups in proteins, metabolites, or materials.
Claims23
A method for detecting native S-nitrosylation within cysteine residues of a protein, comprising:
- providing a biological sample comprising one or more proteins having cysteine residues and a composition comprising a sulfinic acid moiety, wherein the cysteine residues may or may not have a S-nitrosothiol side chain,
- exposing the composition to the biological sample such that an interaction between the sulfinic acid moiety and a S-nitrosothiol side chain of a cysteine residue results in generation of a labeled sulfonothioate moiety, and
- characterizing the cysteine residues of the one or more proteins having sulfonothioate moieties as having undergone S-nitrosylation.
A method for detecting native S-nitrosylation within cysteine residues of a protein, comprising:
- providing a biological sample comprising one or more proteins having cysteine residues and a composition comprising a nitrosothiol moiety, wherein the cysteine residues may or may not have a S-nitrosothiol side chain,
- exposing the composition to the biological sample such that an interaction between the nitrosothiol moiety and a sulfinic acid side chain of a cysteine residue results in generation of a sulfonothioate moiety, and
- characterizing the cysteine residues of the one or more proteins having sulfonothioate moieties as having undergone S-nitrosylation.
Dependencies are inferred from the claim text.