Lentiviral triplex DNA, and vectors and recombinant cells containing lentiviral triplex DNA

A method of expressing a protein of interest in vivo comprising:

• a) administering a lentiviral particle encoding the protein of interest to an individual or to a cell of the individual;
• b) transducing a cell of the individual with the lentiviral particle;
• and
• c) expressing the protein of interest encoded by the lentiviral particle in the cell of the individual in vivo,
• wherein the lentiviral particle comprises:
• at least one copy of lentiviral cPPT and CTS regions;
• a heterologous nucleic acid sequence encoding the protein of interest under the control of an internal promoter not naturally associated with the heterologous nucleic acid sequence;
• a lentiviral LTR that is deleted for the promoter and the enhancer of U3;
• a lentiviral integrase protein;
• a lentiviral reverse transcriptase protein;
• a lentiviral Gag protein;
• and
• an envelope protein.