Compositions and methods for increasing plant growth and yield using rice promoters
AssigneeBenson Hill Biosystems, Inc.
InventorsThomas P. Brutnell, Douglas W. Bryant, Todd Christopher Mockler, Lin Wang
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Abstract
Compositions and methods for improving plant growth are provided herein. Compositions comprise promoter sequences that direct expression of an operably linked nucleotide in a developmentally regulated manner. Polynucleotides, polypeptides, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
Claims19
A method of expressing a gene of interest in a plant comprising transforming a plant cell with a DNA construct comprising a promoter sequence, wherein said promoter sequence comprises the nucleic acid sequence set forth in SEQ ID NO:
- 3, and wherein said promoter sequence is operably linked to a second nucleic acid sequence encoding a functional protein, and regenerating a transformed plant.
A DNA construct comprising, in operable linkage,
- a. a promoter that functions in the plant cell,
- b. a nucleic acid sequence encoding a protein involved in photosynthesis,
- wherein said promoter comprises the nucleotide sequence set forth in SEQ ID NO:
- 3, and wherein said promoter is heterologous to said nucleic acid sequence encoding a protein involved in photosynthesis.
A transformed plant comprising a DNA construct comprising a promoter that functions in a plant cell, wherein said promoter comprises the sequence set forth in SEQ ID NO:
- 3, and wherein said promoter is operably linked to a nucleic acid sequence encoding a protein involved in photosynthesis, and wherein said promoter is heterologous to said nucleic acid sequence encoding a protein involved in photosynthesis.
A method of modulating the growth of a plant comprising inserting into the nuclear genome of a plant cell a promoter sequence upstream of a photosynthetic gene of interest to alter the expression of a said photosynthetic gene, wherein said promoter sequence comprises the sequence set forth in SEQ ID NO:
- 3.
A DNA construct comprising a promoter sequence, said promoter sequence comprising the nucleic acid sequence set forth in SEQ ID NO:
- 3, wherein said promoter sequence is operably linked to a nucleotide of interest encoding a protein involved in photosynthesis and wherein said promoter sequence and said nucleotide of interest are heterologous to each other.
Dependencies are inferred from the claim text.