Use of multi-arm polyethylene glycol modifier and application of multi-arm polyethylene glycol modifier in L-asparaginasum modification
AssigneesZonhon Biopharma Institute Inc., Gensun Institute of Biomedicine Co., Ltd.
InventorsBruce Yong Ma, Jun Wang, Dinglong Wu, Chunlin Xu, Yaofang Wang
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Abstract
Methods for use of a multi-arm polyethylene glycol (PEG) modifier in modification of asparaginase. The described multi-arm PEG modifier enhances the subunit interaction of a multimeric protein to maintain the multimeric protein in a polymerized form, thereby improving the stability of the multimeric protein, maintaining the bioactivity of the multimeric protein, and reducing the probability of exposure of the antigen binding site after depolymerization of the subunits, so as to reduce the immunogenicity.
Claims19
A multimeric protein modified with a multi-arm polyethylene glycol (PEG), the multi-arm polyethylene glycol comprising a chain of repeating units having from 2 to 16 activating groups that react with respective amino acid residues of subunits of the multimeric protein, wherein the activating groups enhance interaction between the subunits such that the multimeric protein modified with the multi-arm PEG has increased stability and reduced immunogenicity as compared with the multimeric protein when modified with a PEG comprising a chain having only 1 of the activating groups.
Dependencies are inferred from the claim text.