Back to results
US 10,400,254 B1Granted
Terpene synthases for biofuel production and methods thereof
AssigneeNational Technology & Engineering Solutions of Sandia, LLC
InventorsWeihua Wu, John Michael Gladden, Benjamin Chiau-Pin Wu, Ryan Wesley Davis
Life of this patent
Estimating the term…
Abstract
The present invention relates to terpene synthases capable of degrading precursors into biofuel compounds, such as terpenoid compounds. In one instance, a transformed organism can include such terpene synthases, as well as vectors encoding such synthases. Methods of employing such synthases and organisms are also described herein.
Claims22
An isolated, genetically engineered organism comprising:
- an exogenous terpenoid precursor, an exogenous enzyme configured to synthesize a terpenoid precursor, or a nucleic acid encoding the exogenous enzyme;
- and
- an exogenous endophytic fungal terpene synthase or a nucleic acid encoding the exogenous endophytic fungal terpene synthase, wherein the exogenous endophytic fungal terpene synthase is selected from the group consisting of a pinene synthase, a guaiene synthase, a pinene and guaiene synthase, a caryophyllene synthase, a chamigrene synthase, a chamigrene and pinene synthase, a gurjunene synthase, a gurjunene and pinene synthase, a gumunene synthase, a selinene synthase, and an isoledene synthase, or a bifunctional synthase of any of these,
- wherein the exogenous endophytic fungal terpene synthase comprises a polypeptide sequence having at least 90% sequence identity to any one of the following SEQ ID NOs or wherein the nucleic acid encoding the exogenous endophytic fungal terpene synthase comprises a nucleic acid sequence encoding a polypeptide sequence having at least 90% sequence identity to any one of the following SEQ ID NOs:
- SEQ ID NO:
- 10, in which X at each position of SEQ ID NO:10 is an amino acid present at a position in one of SEQ ID NOs:11-14 when optimally aligned with SEQ ID NO:10;
- SEQ ID NO:20, in which X at each position of SEQ ID NO:20 is an amino acid present at a position in one of SEQ ID NOs:21-25 when optimally aligned with SEQ ID NO:20;
- SEQ ID NO:30, in which X at each position of SEQ ID NO:30 is an amino acid present at a position in one of SEQ ID NOs:31-34 when optimally aligned with SEQ ID NO:30;
- SEQ ID NO:40, in which X at each position of SEQ ID NO:40 is an amino acid present at a position in one of SEQ ID NOs:41-46 when optimally aligned with SEQ ID NO:40;
- SEQ ID NO:50, in which X at each position of SEQ ID NO:50 is an amino acid present at a position in one of SEQ ID NOs:51-54 when optimally aligned with SEQ ID NO:50;
- or
- SEQ ID NO:60, in which X at each position of SEQ ID NO:60 is an amino acid present at a position in one of SEQ ID NOs:61-64 when optimally aligned with SEQ ID NO:60.
9 more elements
An isolated, genetically engineered organism comprising:
- an exogenous terpenoid precursor, an exogenous enzyme configured to synthesize a terpenoid precursor, or a nucleic acid encoding the exogenous enzyme;
- and
- an exogenous endophytic fungal terpene synthase or a nucleic acid encoding the exogenous endophytic fungal terpene synthase, wherein the exogenous endophytic fungal terpene synthase is selected from the group consisting of a pinene synthase, a guaiene synthase, a pinene and guaiene synthase, a caryophyllene synthase, a chamigrene synthase, a chamigrene and pinene synthase, a gurjunene synthase, a gurjunene and pinene synthase, a gumunene synthase, a selinene synthase, and an isoledene synthase, or a bifunctional synthase of any of these,
- wherein the exogenous endophytic fungal terpene synthase comprises a polypeptide sequence having at least 90% sequence identity to any one of SEQ ID NOs:11-14, 21-25, 31-34, 41-46, 51-54, and 61-64;
- or wherein the nucleic acid encoding the exogenous endophytic fungal terpene synthase comprises a nucleic acid sequence encoding a polypeptide sequence having at least 90% sequence identity to any one of SEQ ID NOs:11-14, 21-25, 31-34, 41-46, 51-54, and 61-64.
2 more elements
An isolated, genetically engineered organism comprising:
- an exogenous terpenoid precursor, an exogenous enzyme configured to synthesize a terpenoid precursor, or a nucleic acid encoding the exogenous enzyme;
- and
- an exogenous endophytic fungal terpene synthase or a nucleic acid encoding the exogenous endophytic fungal terpene synthase, wherein the exogenous endophytic fungal terpene synthase is selected from the group consisting of a pinene synthase, a guaiene synthase, a pinene and guaiene synthase, a caryophyllene synthase, a chamigrene synthase, a chamigrene and pinene synthase, a gurjunene synthase, a gurjunene and pinene synthase, a gumunene synthase, a selinene synthase, and an isoledene synthase, or a bifunctional synthase of any of these,
- wherein the exogenous endophytic fungal terpene synthase comprises a polypeptide sequence having at least 90% sequence identity to XXZZXXZX (SEQ ID NO:71);
- wherein X is any amino acid;
- and wherein Z is selected from the group consisting of Asp, Glu, and His.
3 more elements
An isolated, genetically engineered organism comprising:
- an exogenous terpenoid precursor, an exogenous enzyme configured to synthesize a terpenoid precursor, or a nucleic acid encoding the exogenous enzyme;
- and
- an exogenous endophytic fungal terpene synthase or a nucleic acid encoding the exogenous endophytic fungal terpene synthase, wherein the exogenous endophytic fungal terpene synthase is selected from the group consisting of a pinene synthase, a guaiene synthase, a pinene and guaiene synthase, a caryophyllene synthase, a chamigrene synthase, a chamigrene and pinene synthase, a gurjunene synthase, a gurjunene and pinene synthase, a gumunene synthase, a selinene synthase, and an isoledene synthase, or a bifunctional synthase of any of these,
- wherein the exogenous endophytic fungal terpene synthase comprises a polypeptide sequence having at least 90% sequence identity to XZZXXXSXXZ ZXX (SEQ ID NO:75);
- wherein X is any amino acid;
- and wherein Z is selected from the group consisting of Cys, Asp, Glu, Asn, Gln, Lys, Arg, and absent.
3 more elements
Dependencies are inferred from the claim text.