Mutant CsgG pores
AssigneesOxford Nanopore Technologies PLC, Vib Vzw, Vrije Universiteit Brussel
InventorsStefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Abstract
The invention relates to mutant forms of the outer membrane-located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.
Claims8
An apparatus for characterizing a target analyte, the apparatus comprising a mutant CsgG monomer comprising a variant of the sequence shown in SEQ ID NO:
- 390, wherein the variant comprises a mutation at one or more of positions Y51, N55 and F56, and an in vitro membrane, wherein the monomer is inserted into the in vitro membrane.
An apparatus for characterizing a target analyte, the apparatus comprising a plurality of mutant CsgG monomers, each monomer comprising a variant of the sequence shown in SEQ ID NO:
- 390, wherein each variant comprises a mutation at one or more of positions Y51, N55 and F56, and wherein the plurality of monomers is inserted into a plurality of in vitro membranes.
Dependencies are inferred from the claim text.