High-throughput immune sequencing

A method comprising:

• (a) linking a polynucleotide sequence encoding a heavy chain variable region (VH) and a polynucleotide sequencing encoding a light changing variable region (VL) from a single cell to form paired sequences;
• (b) sequencing a polynucleotide amplified from the paired sequences, thereby forming paired sequence reads;
• (c) selecting a therapeutic antibody candidate comprising a VH and a VL encoded by at least one sequence pair linked in (a);
• and
• (d) screening the therapeutic antibody candidate for a property, wherein the property is selected from the group consisting of functional specificity, affinity and neutralization ability;
• wherein (a) is performed for a plurality of polynucleotide sequences encoding heavy chain variable regions (VH) and a plurality of polynucleotide sequences encoding light chain variable regions (VL) each from a single cell to form a plurality of paired sequences, each representing a sequence pair from an individual cell, and wherein selecting is based on a frequency of each of two or more paired VH and VL sequences from among the plurality of paired sequences and a variance from a reference library for at least one of the two or more paired VH and VL sequences.