Method of producing (-)-rotundone
AssigneeT. Hasegawa Co., Ltd.
InventorsKuniki Kino, Toshiki Furuya
Life of this patent
Estimating the term…
Abstract
The present invention provides a method of producing (−)-rotundone from α-guaiene. The method includes the steps of: (1) allowing a cytochrome P450 protein to act on α-guaiene, which cytochrome P450 belongs to the CYP152 family and is capable of oxidizing the methylene group at position 3 of α-guaiene to the carbonyl group; and/or (2) allowing a cytochrome P450 protein to act on α-guaiene in the presence of an electron transfer protein capable of transferring electrons to the cytochrome P450 protein, which cytochrome P450 belongs to the CYP152, CYP106, or CYP107 family and is capable of oxidizing the methylene group at position 3 of α-guaiene to the carbonyl group.
Claims8
A method of producing (−)-rotundone from α-guaiene, the method comprising the following steps (1) and/or (2):
- (1) allowing a cytochrome P450 protein to act on α-guaiene, which cytochrome P450 (CYP) protein belongs to a CYP152 family and converts α-guaiene to rotundone,
- wherein the cytochrome P450 protein is SEQ ID NO:1, or a protein sequence having a sequence identity of at least 90% to the amino acid sequence of SEQ ID NO:1;
- (2) allowing a cytochrome P450 protein to act on α-guaiene in the presence of an electron transfer protein capable of transferring electrons to the cytochrome P450 protein, which cytochrome P450 (CYP) protein belongs to a CYP152, CYP106, or CYP107 family and converts α-guaiene to rotundone,
- wherein the cytochrome P450 protein is selected from the protein sequences of SEQ ID NOs:1 to 4, or a protein sequence having a sequence identity of at least 90% to an amino acid sequence of any of SEQ ID NOs:1 to 4.
Dependencies are inferred from the claim text.