Back to results
US 10,392,635 B2Granted
Production of tetrahydrocannabinolic acid in yeast
AssigneeLibrede Inc.
InventorsJason L. Poulos, Anthony N. Farnia
Life of this patent
Estimating the term…
Abstract
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
Claims3
A method for making tetrahydrocannabinolic acid, the method comprising:
- transforming S. cerevisiae with a first nucleotide sequence comprising the nucleotide SEQ. ID. NO. 9 or 2 expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- transforming the S. cerevisiae with a second nucleotide sequence comprising the nucleotide SEQ. ID. NO. 5 or 10 expressing olivetolic synthase and expressing olivetolic acid cyclase;
- and
- transforming the S. cerevisiae with a third nucleotide sequence comprising the nucleotide sequence SEQ. ID. NO. 3 expressing aromatic prenyltransferase and expressing tetrahydrocannabinolic acid synthase.
1 more element
A method for making tetrahydrocannabinolic acid, the method comprising:
- transforming S. cerevisiae with a first nucleotide sequence comprising the nucleotide SEQ. ID. NO. 9 or 2 expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- transforming the S. cerevisiae with a second nucleotide sequence comprising the nucleotide SEQ. ID. NO. 5 or 10 expressing olivetolic synthase and expressing olivetolic acid cyclase;
- and
- transforming the S. cerevisiae with a third nucleotide sequence comprising the nucleotide sequence SEQ. ID. NO. 19 expressing aromatic prenyltransferase and expressing tetrahydrocannabinolic acid synthase.
1 more element
A method for making tetrahydrocannabinolic acid, the method comprising:
- transforming S. cerevisiae with a first nucleotide sequence comprising the nucleotide SEQ. ID. NO. 1 expressing an acyl-activating enzyme;
- transforming the S. cerevisiae with a second nucleotide sequence comprising the nucleotide SEQ. ID. NO. 24 expressing a mutant prenyltransferase;
- transforming the S. cerevisiae with a third nucleotide sequence comprising the nucleotide sequence SEQ. ID. NO. 25 expressing olivetolic synthase;
- transforming the S. cerevisiae with a fourth nucleotide sequence comprising the nucleotide SEQ. ID. NO. 26 expressing olivetolic acid cyclase;
- transforming the S. cerevisiae with a fifth nucleotide sequence comprising the nucleotide SEQ. ID. NO. 27 expressing aromatic prenyltransferase;
- and
- transforming the S. cerevisiae with a sixth nucleotide comprising the nucleotide sequence of SEQ. ID. NO. 29 expressing tetrahydrocannabinol (“THC”) synthase.
4 more elements
Dependencies are inferred from the claim text.