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AssigneeJiangnan University
InventorsJingwen Zhou, Jian Chen, Yongkun Lv, Guocheng Du
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The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.
A method for production of pinoresinol using an H2O2 auto-scavenging enzymatic cascade, wherein eugenol is used as a substrate and a whole-cell recombinant Escherichia coli is used as a catalyst to convert eugenol to pinoresinol, wherein the recombinant Escherichia coli over-expresses a vanillyl alcohol oxidase comprising the amino acid sequence of SEQ ID NO:1 and a peroxidase comprising the amino acid sequence of SEQ ID NO:3 or a fusion protein of the vanillyl alcohol oxidase and the peroxidase.
Dependencies are inferred from the claim text.