US 10,392,631 B2 · Fusarium chitin synthase gene chs3b and the use thereof

Abstract

The present invention discloses RNAi constructs derived from a Fusarium chitin synthase gene Chs3b, which has a nucleotide sequence as shown by SEQ ID NO: 1. Five distinct RNAi vectors are constructed for 5 different segments of the Chs3b gene (named Chs3b-1, 2, 3, 4, and 5, respectively), and separately transformed into Fusarium. It is found that siRiNAs in the transformed Fusarium shows a significant inhibition in fungal growth, development, and pathogenicity. The expression of the RNAi vectors against Chs3b in plants may inhibit the infection of Fusarium and improve the resistance of the transgenic plants to Fusarium head blight.

Claims10

Dependent claims

An RNAi construct comprising a fragment of Fusarium chitin synthase gene Chs3b selected from the group consisting of:

Chs3b-1 having the nucleotide sequence of SEQ ID NO:
2, Chs3b-2 having the nucleotide sequence of SEQ ID NO:
3, Chs3b-3 having the nucleotide sequence of SEQ ID NO:
4, Chs3b-4 having the nucleotide sequence of SEQ ID NO:
5, and Chs3b-5 having the nucleotide sequence of SEQ ID NO:6.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/315,124Filed 24 Mar 2015

Priority

CN 2014 1 024003230 May 2014

Published

US 2017/0314040 A12 Nov 2017

Granted

27 Aug 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12N 15/8282
For biotic stress resistance, pathogen resistance, disease resistance: for fungal resistance
C12N 9/1051
Glycosyltransferases: hexosyltransferases
C12N 15/1137
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against enzymes
C12N 15/80
Vectors or expression systems specially adapted for eukaryotic hosts: for fungi

Fusarium chitin synthase gene chs3b and the use thereof

Life of this patent

Fusarium chitin synthase gene chs3b and the use thereof

Life of this patent

Abstract

Claims10