L-arabinose assimilation pathway and uses thereof
AssigneeDeinove
InventorsRemi Bernard, Assia Zigha, Pascale Joseph, Jean-Paul Leonetti
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Abstract
The present invention relates to a new L-arabinose assimilation pathway and uses thereof. In particular, the present invention relates to polypeptides exhibiting L-arabinose isomerase, L-ribulokinase or L-ribulose-5-phosphate-4-epimerase activity, and recombinant host cells expressing said polypeptides. The present invention also relates to a method of producing a fermentation product, preferably ethanol, from an arabinose containing substrate, using a polypeptide or a host cell of the invention.
Claims19
A recombinant host cell comprising a heterologous nucleic acid construct, expression cassette or vector comprising:
- a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 5 and exhibiting L-ribulose-5-phosphate 4 epimerase activity, or a functional fragment thereof, and/or
- a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 1 and exhibiting L-arabinose isomerase activity, or a functional fragment thereof, and/or
- a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 3 and exhibiting L-ribulokinase activity, or a functional fragment thereof,
- wherein the functional fragment is at least 100 contiguous amino acids of said polypeptide, and retains the enzymatic activity of the entire polypeptide.
A recombinant expression cassette or vector, comprising a nucleic acid sequence encoding a polypeptide comprising:
- an amino acid sequence having at least 95% identity to SEQ ID NO:
- 1 and exhibiting L-arabinose isomerase activity, or a functional fragment thereof, or
- an amino acid sequence having at least 95% identity to SEQ ID NO:
- 3 and exhibiting L-ribulokinase activity, or a functional fragment thereof, or
- an amino acid sequence having at least 95% identity to SEQ ID NO:
- 5 and exhibiting L-ribulase-5-phosphate 4 epimerase activity, or a functional fragment thereof,
- wherein the functional fragment is at least 100 contiguous amino acids of said polvpeptide, and retains the enzymatic activity of the entire polypeptide, and
- wherein the nucleic acid sequence is operably linked to a promoter which is heterologous to said nucleic acid sequence.
Dependencies are inferred from the claim text.