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US 10,392,621 B2Granted
Multiaptamer target detection
AssigneeSomaLogic, Inc.
InventorsUrs A. Ochsner, Louis S. Green, Larry Gold, Nebojsa Janjic
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Abstract
Described herein are compositions comprising a first aptamer, second aptamer and a target that are capable of forming a ternary complex, and wherein the first aptamer and the second aptamer comprise C-5 pyrimidine modification schemes that are different, and methods of making and using such compositions.
Claims35
A method for detecting a target in a sample, the method comprising:
- a) contacting the sample with a first aptamer to form a mixture, wherein the first aptamer is capable of binding to the target to form a first complex;
- b) incubating the mixture under conditions that allow for the first complex to form;
- c) contacting the mixture with a second aptamer, wherein the second aptamer is capable of binding the first complex to form a second complex;
- d) incubating the mixture under conditions that allow for the second complex to form;
- e) detecting for the presence or absence of the first aptamer, the second aptamer, the target, the first complex or the second complex in the mixture, wherein the presence of the first aptamer, the second aptamer, the target, the first complex or the second complex indicates that the target is present in the sample;
- and
- wherein, the first aptamer comprises a first C-5 pyrimidine modification scheme, the second aptamer comprises a second C-5 pyrimidine modification scheme, and wherein the first C-5 pyrimidine modification scheme and the second C-5 pyrimidine modification scheme are different,
- further wherein the first C-5 pyrimidine modification scheme comprises a 5-(N-benzylcarboxyamide)-2′-deoxyuridine (BndU).
5 more elements
A method comprising:
- a) contacting a target with a first aptamer to form a mixture, wherein the first aptamer is capable of binding the target to form a first complex;
- b) incubating the mixture under conditions that allow for the first complex to form;
- c) contacting the mixture with a second aptamer, wherein the second aptamer is capable of binding the target to form a second complex;
- d) incubating the mixture under conditions that allow for the second complex to form;
- e) detecting for the presence or absence of the first aptamer and the second aptamer in the mixture, wherein the presence of both the first aptamer and second aptamer in the mixture indicates that the binding of the first aptamer to the target and the binding of the second aptamer to the target is non-competitive;
- and
- wherein, the first aptamer comprises a first C-5 pyrimidine modification scheme, the second aptamer comprises a second C-5 pyrimidine modification scheme, and wherein the first C-5 pyrimidine modification scheme and the second C-5 pyrimidine modification scheme are different.
4 more elements
Dependencies are inferred from the claim text.