Multi-spectral physiologic visualization (MSPV) using laser imaging methods and systems for blood flow and perfusion imaging and quantification in an endoscopic design

A method for multispectral imaging in a multispectral imaging system adapted for an endoscopic system, comprising:

• imaging a sample using a first light source having a first wavelength and being delivered through an endoscopic system;
• imaging the sample using a second light source, different from the first light source, having a second wavelength, different from the first wavelength and being delivered through the endoscopic system;
• receiving information related to the first and second light sources from the sample at a multi-sensor camera having a plurality of sensors, wherein light at the first wavelength is configured to reflect off a surface of the sample into the a first of the plurality of sensors in the multi-sensor camera and light at the second wavelength is configured to penetrate the sample and provide information related to the sample to a second of the plurality of sensors in the multi-sensor camera through the endoscopic system, the light at the first and second wavelengths being aligned and synchronized when provided to the first and second sensors of the multi-sensor camera;
• and
• combining the information related to the first and second light sources received by the multi-sensor camera using at least one processor to image an anatomical structure of the sample, image physiology of blood flow and perfusion of the sample and/or synthesize the anatomical structure and the physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution.

An endoscopic imaging system, comprising:

• an endoscope;
• a light source unit coupled to the endoscope, the light source unit providing first, second and at least a third light source,
• wherein the first light source has a first wavelength configured to image a sample;
• wherein the second light source, different from the first light source, has a second wavelength, different from the first wavelength, configured to image the sample;
• and
• wherein the at least a third light source, different from the first and second light sources, has a third wavelength, different from the first and second wavelengths, configured to image the sample;
• a cameral control unit coupled to the endoscope through a camera head of a multi-sensor camera having a plurality of sensors, the camera head being adapted to receive information related to the first, second and at least third light sources, wherein light at the first wavelength is configured to image a surface of the sample into a first of the plurality of sensors of the multi-sensor camera;
• wherein light at the second wavelength is configured to penetrate the sample to a first depth and provide information related to the sample to a second of the plurality of sensors in the multi-sensor camera;
• and wherein light at the third wavelength is configured to penetrate the sample to a second depth, different from the first depth, and provide information related to the sample to a third sensor of the plurality of sensors in the multi-sensor camera, the information related to the first, second and third wavelengths being aligned and synchronized when provided to the first, second and third sensors of the multi-sensor camera;
• and
• an image processing unit coupled to endoscope configured to combine the information related to the first, second and at least third light sources provided by the camera head to image an anatomical structure of the sample, image physiology of blood flow and perfusion of the sample and/or synthesize the anatomical structure and the physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution.