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US 10,386,290 B2Granted
Apparatuses, systems and methods for imaging flow cytometry
AssigneeLife Technologies Corporation
InventorsGregory Kaduchak, Michael Ward
Life of this patent
Estimating the term…
Abstract
The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
Claims19
A system, comprising:
- a flow channel,
- the flow channel having an inlet, and
- the flow channel defining a flow path for fluid communicated therethrough;
- a first optical train comprising a first illumination source and a first detection module,
- the first detection module being disposed so as to receive a signal related to illumination of a first region of the flow channel by the first illumination source, the first region being located at a first distance from the inlet of the flow channel;
- a second optical train comprising a second illumination source and a second detection module,
- the second detection module being disposed so as to receive a signal related to illumination of (i) a second region of the flow channel or of (ii) a region of a second flow channel by the second illumination source, the second region or the region of the second flow channel being located at a second distance from the inlet of the flow channel, the second distance being greater from the first distance, and
- the system further comprising a field module,
- the field module being configured to increase or reduce a field at a region of the flow channel so as to move at least some particles in a flow in the flow channel and sensitive to the field from a velocity streamline So of particle velocity within the flow in the flow channel to a velocity streamline S1 of particle velocity within:
- (i) the flow in the flow channel or (ii) a flow in the second flow channel, wherein the particle velocity at So during system operation differs from the particle velocity at S1.
7 more elements
A method, comprising:
- flowing a population of particles through a flow channel;
- operating a field module configured to increase or reduce a field at a region of the flow channel so as to move at least some of the population of particles sensitive to the field from a velocity streamline So of particle velocity within the flow in the flow channel to a velocity streamline S1 of particle velocity (i) within the flow in the flow channel or (ii) within a second flow channel, wherein the particle velocity at So during system operation differs from the particle velocity at S1, between the first and second regions of the flow channel
- with a first optical train, illuminating at least some of the population of particles at a first region of the flow channel and collecting a first signal or lack thereof related to the illuminating;
- with a second optical train, illuminating at least some of the population of particles at (i) a second region of the flow channel or (ii) a region of the second flow channel and collecting a second signal or lack thereof related to the illuminating,
- a particle velocity at the first region of the flow channel differing from a particle velocity at (i) the second region of the flow channel or (ii) the region of the second flow channel, and wherein the first signals differs from the second signal.
2 more elements
A method, comprising:
- communicating a population of particles through a flow channel, in a direction from a first region of the flow channel toward a second region of the flow channel;
- with a first optical train, illuminating at least some of the population of particles at the first region of the flow channel and collecting a first signal related to the illuminating;
- with a second optical train, illuminating at least some of the population of particles at the second region of the flow channel and collecting a second signal related to the illuminating,
- increasing or reducing a field on a subset of the particles between the first and second regions of the flow channel,
- the field being increased or reduced so as to move the subset of particles from a velocity streamline So of particle velocity within the flow channel to a velocity streamline S1 of particle velocity within the flow channel, wherein the particle velocity at So differs from the particle velocity at S1.
2 more elements
Dependencies are inferred from the claim text.