Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
AssigneeOxford Nanopore Technologies PLC
InventorsAndrew John Heron, Clive Gavin Brown, Rebecca Victoria Bowen, James White, Daniel John Turner, Joseph Hargreaves Lloyd, Christopher Peter Youd
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Abstract
The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also relates to new methods of characterizing target polynucleotides.
Claims20
A method for attaching one or more polynucleotide binding proteins to a target polynucleotide, comprising:
- (a) providing the one or more polynucleotide binding proteins bound to one or more loading moieties;
- and
- (b) covalently attaching the one or more loading moieties to the target polynucleotide.
A kit for covalently attaching one or more polynucleotide binding proteins to a target polynucleotide, comprising (a) the one or more polynucleotide binding proteins bound to one or more loading moieties and (b) a ligase.
Dependencies are inferred from the claim text.