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US 10,385,375 B2Granted
Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms
AssigneeNovozymes A/S
InventorsChristine Lang, Markus Veen
Life of this patent
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Abstract
The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
Claims21
A method for preparing 7-dehydrocholesterol and/or cholesterol, the method comprising the steps of:
- culturing a yeast organism which, compared to the wild type, has an increased activity of at least one of the activities selected from the group consisting of:
- Δ8-Δ7-isomerase activity of a Δ8-Δ7-isomerase having the amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 2,
- Δ5-desaturase activity of a Δ5-desaturase having the amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 8;
- and
- Δ24-reductase activity of a Δ24-reductase having the amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 14;
- and wherein the yeast organism additionally have increased HMG-CoA-reductase activity of HMG-CoA-reductase having the amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 24;
- harvesting the yeast after culturing;
- and
- isolating the 7-dehydrocholesterol and/or cholesterol after harvesting.
10 more elements
Dependencies are inferred from the claim text.