Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product

A method of producing a rubber, the method comprising:

• a step of binding a protein expressed by a gene coding for a cis-prenyltransferase (CPT) family protein, a protein expressed by a gene coding for a Nogo-B receptor (NgBR) family protein, and a protein expressed by a gene coding for a rubber elongation factor (REF) family protein to rubber particles in vitro,
• wherein the gene coding a for a cis-prenyltransferase (CPT) family protein, the gene coding for a Nogo-B receptor (NgBR) family protein, and the gene coding for a rubber elongation factor (REF) family protein are each derived from plants, and
• wherein the cis-prenyltransferase (CPT) family protein is at least one selected from the group consisting of CPT from Hevea brasiliensis (HRT1, HRT2, CPT3-5), AtCPTl-9 from Arabidopsis thaliana, CPT 1-3 from lettuce, and CPT 1-3 from Taraxacum kok-saghyz, and wherein the Nogo-B receptor (NgBR) family protein is at least one selected from the group consisting of NgBR from Hevea brasiliensis (HRTBP), LEW1 from Arabidopsis thaliana, LsCPTLl-2 from lettuce, and TbRTA from Taraxacum, and
• wherein the rubber elongation factor (REF) family protein is REF from Hevea brasiliensis.