Back to results
US 10,385,361 B2Granted
Production of manool
AssigneeFirmenich SA
InventorsMichel Schalk, Letizia Rocci
Life of this patent
Estimating the term…
Abstract
Provided herein are methods of producing (+)-manool comprising: contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2; and contacting the CPP with a sclareol synthase enzyme to form (+)-manool.
Claims5
A method of producing (+)-manool comprising:
- a. contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to SEQ ID NO:
- 1 or SEQ ID NO:2;
- and
- b. contacting the (9S, 10S)-copalyl diphosphate CPP with a sclareol synthase enzyme to form (+)-manool;
- and
- c. optionally isolating the (+)-manool.
3 more elements
Dependencies are inferred from the claim text.