Generation of CTL lines with specificity against multiple tumor antigens or multiple viruses
AssigneesBaylor College of Medicine, Wilson Wolf Manufacturing
InventorsAnn Marie Leen, Ulrike Gerdemann, Cliona M. Rooney, Juan F. Vera Valdes, John R. Wilson
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Abstract
The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and/or that employ a particular bioreactor having a gas permeable membrane.
Claims15
A method of generating antigen-specific T lymphocytes that recognize at least one antigen from two or more different viruses, comprising the step of:
- directly stimulating PBMCs comprising antigen-specific T lymphocytes that recognize at least one epitope from a viral antigen of at least two different viruses with more than one library of peptides, wherein peptides in each library comprise a sequence of amino acids spanning all or part of at least one epitope of at least one antigen of each different virus, thereby expanding antigen-specific T lymphocytes capable of recognizing a cell that expresses part or all of at least one antigen from the different viruses, wherein the antigen-specific T lymphocytes comprise CD4+ T-lymphocytes and CD8+ T-lymphocytes.
Dependencies are inferred from the claim text.