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US 10,384,189 B2Granted
Functionalized surfaces and preparation thereof
AssigneeTwist Bioscience Corporation
InventorsBill James Peck, Pierre Indermuhle, Eugene P. Marsh, Andres Fernandez, David Stern
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Abstract
Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.
Claims22
A method for surface patterning, the method comprising:
- applying a first set of molecules to a surface of a structure, wherein each of the first set of molecules comprises a reactive group capable of binding to a nucleoside;
- applying electromagnetic radiation (EMR) to predetermined regions of the surface, wherein the EMR comprises a wavelength from about 100 nm to about 300 nm, wherein application of the EMR results in removal of the first set of molecules at the predetermined regions, thereby defining different loci for oligonucleotide extension;
- and
- synthesizing at least 100 different oligonucleotides base by base, wherein each oligonucleotide extends from a different locus, and wherein the different loci are at least about 75% uniform when measured by calculating amplitude of signal variation for oligonucleotides extending from each locus divided by total signal intensity following white light illumination using an optical microscope.
1 more element
Dependencies are inferred from the claim text.