Method for producing a plurality of measurement regions on a chip, and chip with measurement regions
AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsMarkus Schieber, Heinz Schoeder
Life of this patent
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Abstract
A chip and a method for producing the chip with a plurality of measurement regions which are provided with electrodes for electrically detecting reactions in which, in order to reliably separate the individual measurement regions from one another, a monolayer of a fluorosilane is formed on the chip surface which has strongly hydrophobic properties. Therefore, during spotting with a liquid, the drops of liquid applied by spotting can be reliably prevented from coalescing, and thus, causing mixing of the substances in the drops of liquid which are supposed to be immobilized in the measurement regions.
Claims15
A chip comprising a plurality of electrically addressable measurement regions, wherein a compartmental structure separates the measurement regions from one another on the chip surface, wherein the compartmental structure is formed from a self-assembling hydrophobic monolayer of a silane compound which covers the chip surface outside the measurement regions
- wherein the measurement regions have hydrophilic properties,
- wherein the measurement regions have an average diameter of more than 100 μm and less than 500 μm,
- wherein the compartmental structure is smaller in height than in width, and
- wherein the width of the compartmental structure between two adjacent measurement regions is greater than the height of the compartmental structure relative to the chip surface carrying the measurement regions by at least a factor 5.
A method for producing a chip having a plurality of electrically addressable measurement regions or for producing a plurality of measurement regions on a chip, comprising:
- structuring electrically contactable electrode pairings on the chip in each of the electrically addressable measurement regions, the measurement regions being formed by producing a compartmental structure which separates the measurement regions from one another, and
- producing hydrophobic wetting properties on a chip surface outside of the measurement regions
- wherein the compartmental structure is formed from the self-assembling hydrophobic monolayer of a silane compound which covers the chip surface outside of the measurement regions, and
- producing hydrophilic properties in the measurement regions
- wherein the measurement regions have an average diameter of more than 100 μm and less than 500 μm,
- wherein the compartmental structure is smaller in height than in width, and
- wherein the width of the compartmental structure between two adjacent measurement regions is greater than the height of the compartmental structure relative to the chip surface carrying the measurement regions by at least a factor 5.
Dependencies are inferred from the claim text.