Illumination setting method, light sheet microscope apparatus, and recording medium

An illumination setting method comprising:

• acquiring, by a computer and from an imaging device, an image of a sample onto which a light sheet has been radiated by an illumination optical system;
• determining, by the computer and based on the image of the sample that has been acquired from the imaging device, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system, wherein the subordinate ray angle is a maximum angle formed by an optical axis on an exit side of the illumination optical system, and the subordinate ray angle with respect to the width direction is in a cross-section that includes the width direction and a traveling direction of the light sheet;
• and
• outputting, by the computer, a control signal that gives an instruction to perform a setting of the illumination optical system that corresponds to the determined subordinate ray angle.

A light sheet microscope apparatus comprising:

• an illumination optical system that radiates a light sheet onto a sample;
• an imaging device that acquires an image of the sample onto which the light sheet has been radiated by the illumination optical system;
• a controller that determines, based on the image of the sample that has been acquired by the imaging device, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system, wherein the subordinate ray angle is a maximum angle formed by an optical axis on an exit side of the illumination optical system, and the subordinate ray angle with respect to the width direction is in a cross-section that includes the width direction and a traveling direction of the light sheet;
• and
• a setting device that performs a setting of the illumination optical system according to the subordinate ray angle determined by the controller.

A non-transitory recording medium having stored therein a program that causes a computer to execute a process comprising:

• acquiring, from an imaging device, an image of a sample onto which a light sheet has been radiated by an illumination optical system;
• determining, based on the image of the sample that has been acquired from the imaging device, a subordinate ray angle with respect to a width direction of the light sheet emitted from the illumination optical system, wherein the subordinate ray angle is a maximum angle formed by an optical axis on an exit side of the illumination optical system, and the subordinate ray angle with respect to the width direction is in a cross-section that includes the width direction and a traveling direction of the light sheet;
• and
• outputting a control signal that gives an instruction to perform a setting of the illumination optical system that corresponds to the determined subordinate ray angle.